Journal: bioRxiv
Article Title: Canalization of circuit assembly by δ-protocadherins in the zebrafish optic tectum
doi: 10.1101/2025.01.29.635523
Figure Lengend Snippet: a. Schematic of the experimental setup. A 6dpf larva is embedded in agarose in an imaging chamber, allowing 104° view of a projection screen. b. The visual stimuli are presented to the right eye and the neural responses are visualized in the contralateral (left) hemi-tectum. c. The stimulation protocol is a series of 5s on 10s off of a moving sinusoidal grating, 12 directions spaced at 30° intervals to encompass the full 360°. This is repeated three times. Below is a lateral view of the head of a zebrafish larva. The inset shows the directions of motion of sinusoidal gratings with reference to the rostro-caudal axis of the fish. The direction color-code is used throughout the paper. d. Image stacks consisting of 7 optical sections spaced at 10 μm were collected at 1s intervals. Shown are average intensity images of individual planes from a timelapse sequence collected in a 6 dpf transgenic larva, Tg(elav3l:HistoneH2B-GCaMP8s). The visually-responsive hemi-tectum is outlined in yellow. e. Example data showing all the fluorescence traces from visually-responsive neurons collected from one larva. f. ΔF/F traces selected from those shown in e . g. Responses were averaged across the three trials. Shown are the trial averages from the traces in f .
Article Snippet: The plasmid Tol2-elavl3:H2B-GCaMP8s was assembled using the following plasmids: Tol2-elavl3-GCaMP6s (a gift from Misha Ahrens; Addgene plasmid # 59530; http://n2t.net/addgene:59530 ; RRID:Addgene_59530), CMV:Histone H2B-mGL (H2B-mGreenLantern was a gift from Gregory Petsko; Addgene plasmid # 164464; http://n2t.net/addgene:164464 ; RRID:Addgene_164464), and CMV:jGCaMP8s (gift from GENIE Project; Addgene plasmid # 162371; http://n2t.net/addgene:162371 ; RRID:Addgene_162371).
Techniques: Imaging, Sequencing, Transgenic Assay, Fluorescence
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